Fig. 4

CGS-21680 effect on interleukin (IL)-2 and IL-10 release and on nuclear factor (NF)-kB activation. Effect of CGS-21680 (100 nM and 1 μM) and SCH 442416 (1 μM) in cultured lymphocytes of patients with systemic lupus erythematosus (SLE) (n = 20) and healthy subjects (n = 20) stimulated by lipopolysaccharide (LPS) (0.1 mg/ml) or phorbol 12-myristate 13-acetate (PMA) (2 ng/ml) and ionomycin (0.2 μM) on (a) IL-2 release, (b) IL-10 release, and (c) NF-kB activation. Data are expressed as the mean ± SEM of three independent experiments performed in triplicate * p < 0.01 versus LPS-treated cells by one-way analysis of variance with Dunnett’s test