Fig. 3

Lesinurad inhibits URAT1 and OAT4 urate-transport activity with equal potency, and does not inhibit GLUT9. Urate transport was measured in cells expressing URAT1-553G (closed circles) or OAT4-551G (triangles) treated with different amounts of lesinurad (a) (left panel), benzbromarone (b) (center panel), or probenecid (c) (right panel). Untransfected cells (b) (open circles) were used to reveal the background uric acid transport in the cells. Results are from a single experiment and are representative of multiple experiments. Each point is from samples performed in triplicate, presented as mean ± SEM. d Lesinurad does not inhibit GLUT9. Urate transport was measured in oocytes injected with water and treated with dimethyl sulfoxide (DMSO) (open bar), or in oocytes injected with cRNA expressing GLUT9 (SLC2a9v2/GLUT9ΔN) (solid bars) and either treated with DMSO, 100 μM benzbromarone, or 100 μM lesinurad. Benzbromarone inhibited GLUT9, as shown previously [21, 55]. Results are shown in mean ± SEM. SLC2A9v1/GLUT9 was also examined, yielding similar results. UA uric acid