Catabolic and proinflammatory effects of leptin in chondrocytes are regulated by suppressor of cytokine signaling-3

Table 1 Primer and probe sequences for quantitative RT-PCR

Gene	Oligunucleotide	Sequence	Conc. (nM)
	Forward primer	GCATGGCCTTCCGTGTTC	300
Mouse GAPDH	Reverse primer	GATGTCATCATACTTGGCAGGTTT	300
	Probe	TCGTGGATCTGACGTGCCGCC	150
	Forward primer	GCGGGCACCTTTCTTATCC	300
Mouse SOCS-3	Reverse primer	AAGCTGCCCCCCTCACA	300
	Probe	CTCGGACCAGCGCCACTTCTTCA	150
	Forward primer	CCTGGTACGGGCATTGCT	300
Mouse iNOS	Reverse primer	GCTCATGCGGCCTCCTT	300
	Probe	CAGCAGCGGCTCCATGACTCCC	150
	Forward primer	TCGGAGGCTTAATTACACATGTTC	900
Mouse IL-6	Reverse primer	CAAGTGCATCATCGTTGTTCATAC	300
	Probe	CAGAATTGCCATTGCACAACTCTTTTCTCA	200
	Forward primer	TTGTGTTTGCAGAGCACTACTTGA	900
Mouse MMP-13	Reverse primer	AACTGTGGAGGTCACTGTAGACTTCTT	900
	Probe	CATCCTGCGACTCTTGCGGGAATC	250

SOCS-3 suppressor of cytokine signaling-3, iNOS inducible nitric oxide synthase, IL-6 interleukin-6, MMP-13 matrix metalloproteinase-13, Conc. concentration

ISSN: 1478-6362