Fig. 1
From: A novel DNA/histone H4 peptide complex detects autoantibodies in systemic lupus erythematosus sera
Histone 4 peptides interacting with plasmid DNA. Peptides were pre-incubated with plasmid DNA, linearized by means of EcoRI digestion, and the mix was loaded on agarose gel. The H4(14-34) and H4(14-22) peptides, but not H2b and control peptides, retard the migration of linearized plasmid DNA (a). The H4(14-34) peptide retards the migration of plasmid DNA in a concentration-dependent manner (b). a. Lane 1: MW marker 1 Kbp; Lane 2: pPK201/CAT, unrestricted; Lane 3: pPK201/CAT, restricted; Lane 4: restricted pPK201/CAT + H2B(107-125); Lane 5: restricted pPK201/CAT + H4(56-71); Lane 6: restricted pPK201/CAT + H4(76-91)-Gly; Lane 7: restricted pPK201/CAT + H4(7-22); Lane 8: restricted pPK201/CAT + H4(31-50); Lane 9: restricted pPK201/CAT + H4(14-34); Lane 10: restricted pPK201/CAT + H4(21-37); Lane 11: restricted pPK201/CAT + MAP-Cys-[H4(14-34)]4; Lane 12: restricted pPK201/CAT + H4(14-22); Lane 13: restricted pPK201/CAT + scramble H4(14-34); Lane 14: restricted pPK201/CAT + FcεRIα(46-65). b: Lane 1: MW markers 1 Kbp; Lane 2: PK plasmid, unrestricted; Lane 3: PK plasmid, restricted; Lane 4: PK plasmid, restricted + H4 peptide, 5 μg; Lane 5: PK plasmid, restricted + H4 peptide, 2.5 μg; Lane 6: PK plasmid, restricted + H4 peptide, 1.25 μg; Lane 7: PK plasmid, restricted + H4 peptide, 0.625 μg