Fig. 1

Transforming growth factor-ß (TGF-ß) down-regulated basal A20 expression and prevented its induction. Confluent foreskin fibroblasts were incubated with TGF-ß2 (10 ng/ml or indicated concentrations) or ultrapure lipolysaccharide (LPS) (500 ng/ml) for 24 h or indicated periods. RNA and whole cell lysates were examined by qPCR (a, b (left panels), c) and western analysis (a, b (right panels), d). Representative immunoblots (n = 3). Band intensities, normalized for beta actin or tubulin in each lane are shown below. qPCR results, normalized with glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are means ± SD of triplicate determinations (n = 3); *p < 0.05