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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: A20 suppresses canonical Smad-dependent fibroblast activation: novel function for an endogenous inflammatory modulator

Fig. 3

A20 blocks intracellular canonical transforming growth factor-ß (TGF-ß)-Smad signaling. Foreskin fibroblasts transiently transfected with A20 or empty vector were incubated with TGF-ß2 (10 ng/ml) for 24 h. a Whole cell lysates were examined by western analysis (n = 2). b Immunofluorescence confocal microscopy using antibody to Smad2/3 (green color). Nuclei identified by 6-diamidino-2-phenylindone (DAPI) (blue). Bar = 25 μm. c Fibroblasts were transiently co-transfected with [SBE]4-luc and A20 (50 ng) in absence or presence of TGF-ß2 along with Renilla luciferase pRL-TK. Following a 24-h incubation, whole-cell lysates were collected and assayed for their luciferase activity. Results are means ± SD of triplicate determinations (n = 2)

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