Fig. 4

A20 attenuates toll-like receptor 4 (TLR4)-dependent fibrotic responses. Fibroblasts transiently transfected with A20 or empty vector were incubated with ultrapure lipolysaccharide (LPS) and/or transforming growth factor-ß2 (TGF-ß2) (10 ng/ml). Following a 24-h incubation, RNA isolated and was examined by qPCR; results, normalized with glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are means ± SD of triplicate determinations (n = 2)