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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: A20 suppresses canonical Smad-dependent fibroblast activation: novel function for an endogenous inflammatory modulator

Fig. 6

Adiponectin induces A20 gene expression in fibroblasts. Foreskin fibroblasts were incubated with ultrapure lipolysaccharide (LPS) (500 ng/ml, 30 minutes) (lane 5, left panel) and/or recombinant adiponectin (APN) (10 μg/ml in left panels or as indicated in the right panel) for 0–45 minutes or as indicated. Left panel, levels of RNA determined by qPCR. Results, normalized with glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are means ± SD of triplicate determinations (n = 2). Right panels, western analysis of whole-cell lysates. Representative immunoblots (n = 2). Band intensities, normalized for tubulin in each lane are shown below

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Arthritis Research & Therapy

ISSN: 1478-6362

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