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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Glucocorticoid-induced leucine zipper (GILZ) is involved in glucocorticoid-induced and mineralocorticoid-induced leptin production by osteoarthritis synovial fibroblasts

Fig. 1

Glucocorticoid-induced leucine zipper (GILZ) expression is induced by prednisolone and aldosterone through glucocorticoid receptor (GR). a Human osteoarthritis (OA) synovial fibroblasts were pre-incubated or not for 1 h with a GR inhibitor (mifepristone) or mineralocorticoid receptor (MR) inhibitors (eplerenone and spironolactone) and were then stimulated for 5 days with a glucocorticoid (prednisolone) or a mineralocorticoid (aldosterone). GILZ and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression in whole-cell extracts were analyzed by western blotting. Right panels, quantification results of western blots shown in left panels. Protein levels were normalized to GAPDH. Graphs represent mean +/- SD (n = 4 patients). Significance was set at p < 0.05. b*Significantly different from a; c*significantly different from b; d not significantly different from b. b, c Synovial fibroblasts were infected with three different lentiviruses expressing GR shRNA or with a non-target control lentivirus and were stimulated or not for 5 days with prednisolone or aldosterone. A GR inhibitor (mifepristone) was used as a positive control for GR inactivation. GILZ, GR, and GAPDH expression in whole-cell extracts were analyzed by western blotting. Right (b) and upper (c) panels, quantification results of western blots shown in left panels and lower panels. Protein levels were normalized to GAPDH. Graphs represent mean +/- SD (n = 3 patients). Significance was set at p < 0.05. f*Significantly different from e; h*significantly different from g; i*significantly different from h. kD kiloDalton, shRNA short hairpin RNA

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