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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Glucocorticoid-induced leucine zipper (GILZ) is involved in glucocorticoid-induced and mineralocorticoid-induced leptin production by osteoarthritis synovial fibroblasts

Fig. 3

Leptin secretion and leptin receptor (Ob-R) expression were induced by prednisolone and aldosterone through glucocorticoid receptor (GR) signaling. Synovial fibroblasts were infected with three different lentiviruses expressing GR short hairpin RNA (shRNA) or with a non-target control lentivirus and were stimulated for 5 days with prednisolone or aldosterone. The GR inhibitor mifepristone was used as a positive control for GR inhibition. Leptin expression was measured in the cell culture supernatants by ELISA. Ob-R and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression were analyzed in whole-cell extracts by western blotting. Middle panel, quantification results of western blots shown in the lower panel. Protein levels were normalized to GAPDH. Graphs represent mean +/- SD (n = 3 patients). Significance was set at p < 0.05. b*Significantly different from a; c*significantly different from b*. kD kiloDalton

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