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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Glucocorticoid-induced leucine zipper (GILZ) is involved in glucocorticoid-induced and mineralocorticoid-induced leptin production by osteoarthritis synovial fibroblasts

Fig. 6

Transforming growth factor-β (TGF-β) decreased both prednisolone-induced leptin secretion and glucocorticoid-induced leucine zipper (GILZ) expression; Compound A (CpdA) did not induce GILZ expression. Human osteoarthritis (OA) synovial fibroblasts were stimulated for 5 days with prednisolone, TGF-β (A), or CpdA (B). Leptin expression was measured in the cell culture supernatants by ELISA. GILZ and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression in whole-cell extracts was analyzed by western blotting. Right panels, quantification results of western blots shown in the left panels. Protein levels were normalized to GAPDH. Graphs represent mean +/- SD (n = 3 patients). b*Significantly different from a; c*significantly different from b; e*significantly different from d; f not significantly different from d

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