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Fig. 7 | Arthritis Research & Therapy

Fig. 7

From: Glucocorticoid-induced leucine zipper (GILZ) is involved in glucocorticoid-induced and mineralocorticoid-induced leptin production by osteoarthritis synovial fibroblasts

Fig. 7

Glucocorticoid-induced leucine zipper (GILZ) silencing inhibited glucocorticoid-induced and mineralocorticoid-induced leptin and leptin receptor (Ob-R) expression. Human osteoarthritis (OA) synovial fibroblasts were infected with three different lentiviruses expressing GILZ short hairpin RNA (shRNA) or with a control lentivirus (multiplicity of infection (MOI) 30 for a and b; MOI 5, 10 and 20 for c). After 72 h, the cells were stimulated for 5 days with a glucocorticoid (1 μM prednisolone) or mineralocorticoid (10 μM aldosterone). Leptin expression was measured in the cell culture supernatants by ELISA. Ob-R, GILZ and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression in whole-cell extracts was analyzed by western blotting. Right panels, quantification results of western blots shown in the left panels. Protein levels were normalized to GAPDH. Graphs represent mean +/- SD (n = 5 patients). b*Significantly different from a; c*significantly different from b. kD kiloDalton

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