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Fig. 8 | Arthritis Research & Therapy

Fig. 8

From: Glucocorticoid-induced leucine zipper (GILZ) is involved in glucocorticoid-induced and mineralocorticoid-induced leptin production by osteoarthritis synovial fibroblasts

Fig. 8

Glucocorticoid-induced leucine zipper (GILZ) silencing did not alter prednisolone-induced glucocorticoid receptor (GR) degradation. Human osteoarthritis (OA) synovial fibroblasts were infected with a lentivirus expressing GILZ short hairpin RNA (shRNA) or with a control lentivirus. After 72 h, the cells were stimulated for 1, 4, 6, or 12 h with a glucocorticoid (1 μM prednisolone). Leptin expression was measured in the cell culture supernatants by ELISA. GILZ and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression in whole-cell extracts was analyzed by western blotting. Upper panel, quantification results of western blot shown in the lower panel. Protein levels were normalized to GAPDH. Graph represents mean +/- SD (n = 3) patients. kD kiloDalton

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