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Fig. 9 | Arthritis Research & Therapy

Fig. 9

From: Glucocorticoid-induced leucine zipper (GILZ) is involved in glucocorticoid-induced and mineralocorticoid-induced leptin production by osteoarthritis synovial fibroblasts

Fig. 9

Glucocorticoid-induced leucine zipper (GILZ) inhibition did not alter the capacity for prednisolone to reduce IL-6, IL-8 and matrix metalloproteinase-1 (MMP-1) production. Human osteoarthritis (OA) synovial fibroblasts were infected with three different lentiviruses expressing GILZ short hairpin RNA (shRNA) or with a control lentivirus (one sample was infected with one shRNA and the three results were pooled). Cells were pre-incubated (D, E, F) or not (A, B, C) for 1 h with prednisolone (1 μM) and were then stimulated or not with TNF-α (10 ng/mL) for 12 h. The IL-6, IL-8 and MMP-1 levels in the cell culture supernatants were measured by ELISA. Graphs represent mean +/- SD (n = 3 patients). Fold reductions in IL-6, IL-8, and MMP-1 induced by TNF-α were measured by comparing the levels in the presence or not of prednisolone (1 μM). b*Significantly different from a; c not significantly different from b; e f not significantly different from d

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