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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: S100A8/A9, a potent serum and molecular imaging biomarker for synovial inflammation and joint destruction in seronegative experimental arthritis

Fig. 3

Serum S100A8/A9 levels correlate with histological parameters of inflammation and local synovial S100A8 and S100A9 levels. a Increased joint swelling was associated with increased thickening of the synovium (black line) and serum levels of S100A8/A9 correlated with the cell influx in the synovium of 16-week-old IL-1Ra–/– mice. b IL-1Ra–/– mice with increased macroscopic score for joint swelling showed a clear increased S100A8 and S100A9 expression, with similar distribution, in the infiltrating cells of the ankle joint. Expression of S100A8 and S100A9 within the inflamed ankle joint of IL-1Ra–/– mice is co-expressed in monocytes and neutrophils. Isotype control IgG staining for S100A8 and S100A9 staining showed no staining in ankle joint sections of arthritic IL-1Ra–/– mice (inserts). c Intravenous injection of polyclonal anti-S100A8-Cy7 in 16-week-old arthritic IL-1Ra–/– mice (n = 6) led to a significantly increased fluorescent signal in the ankle joints compared with mice injected with irrelevant Rab-IgG-Cy7 (n = 6) (P = 0.0002) (macroscopic score for joint swelling in white). d Anti-S100A8-Cy7 targeting was imaged in ankle joints of IL-1Ra–/– mice with various degrees of joint swelling and the observed fluorescent signal correlated with the macroscopic score for joint swelling. ***P < 0.001. Ca calcaneus, Ig immunoglobulin, IL-1Ra interleukin-1 receptor antagonist, Nav navicular bone, Ta talus

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