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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Bone loss and aggravated autoimmune arthritis in HLA-DRβ1-bearing humanized mice following oral challenge with Porphyromonas gingivalis

Fig. 2

Generation of antibodies to citrullinated protein antigens is enhanced in mice bearing the transgene for human HLA-DR1β, and requires PAD enzyme. Healthy suspensions of live P. gingivalis or a PADnull mutant strain (dPAD) were mixed with CMC and brushed onto the gingival tissues of dual-reporter B6.DR1 mice or WT C57BL/6 mice. Serum samples were obtained 3, 4, and 6 weeks after brushing and applied to commercial ACPA ELISA according to the manufacturer’s instructions. Unbrushed mice generated no detectable ACPAs. The supplied human secondary detection reagent was replaced with an anti-mouse IgG2b antibody detection reagent suggested by the manufacturer. N = 5 for each group. Error bars = SEM. ACPA anti-citrullinated protein antibody, OD optical density

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