Fig. 3

Prevention of splenomegaly by high-dose irinotecan (Irino). Spleens from 18 week-old MRL/lpr mice treated with saline (n = 7), 25 mg/kg irinotecan (n = 6), or 1 mg/kg irinotecan (n = 6) were analyzed by flow cytometry. MRL/MpJ mice treated with saline (n = 7) were used as controls (Ctrl). a Representative image of the spleen from each treatment group (left) and the spleen weights (right). b Representative bivariate contour plots from each treatment group showing the subpopulation of T cells double negative for CD4 and CD8 and positive for CD3 and B220 (DN T cells). Cells were gated for live, singlets, and negative for CD4 and CD8 expression. (Right) Total number of splenic DN T cells. c (Top left) Numbers of CD3^–B220⁺CD138^– B cells and (top right) the expression of CD69. (Bottom left) Numbers of CD3^–B220⁺CD138⁺ plasmablasts and (bottom right) CD3^–B220^–CD138⁺ plasma cells. Each symbol represents an individual mouse; bars show mean ± SEM.*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, versus saline-treated MRL/lpr mice by one-way analysis of variance with Bonferroni post hoc test