Fig. 4

GMSC transfer therapy influences the polarization of the Th cells. a Representative flow cytometry data show the expression of CD25 and FoxP3 gated on CD4 cells (regulatory T cells; Tregs) in the spleens and DLNs of four groups of CIA mice. b WT GMSCs (GMSC-WT) and FasL TF GMSCs (FasL TF) significantly decreased the percentage of CD4⁺INF-γ⁺ Th1 and CD4⁺IL-17⁺ Th17 cells in the mouse DLN and spleen, and increased the percentage of CD4⁺CD25⁺FoxP3⁺ Treg, whereas FasL^–/– GMSCs (FasL ^–/–) did not significantly alter the frequencies of the subset Th cells in the CIA mice. n = 6 in each group.*P < 0.05, **P < 0.01. Values are given as mean ± SEM. IL interleukin, LN lymph node, N.S. not significant