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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: p53 predominantly regulates IL-6 production and suppresses synovial inflammation in fibroblast-like synoviocytes and adjuvant-induced arthritis

Fig. 2

p53 did not induce apoptosis in FLS or the synovium (a–d), but did have control over cell growth (eh). a Trypan blue staining 3 days following transfection with Ad-LacZ and Ad-p53 at different multiplicity of infection (MOI). b MTT assay 3 days following transfection with Ad-LacZ and Ad-p53 at different MOI. c Cell cycle in FLS following transfection with Ad-p53 (MOI = 100) on day (d)3, d5, and d7. d TUNEL staining on slides of collected FLS (left) and synovium 3 days following Ad-p53 treatment (right). e Deletion of p53 in FLS increased cell growth as measured by Alamar blue staining. *,p<0.05 f Deletion of p53 in FLS contribute to the lower expression of p21 thereafter. g Bax expression following knockdown of p53 was analyzed by Western blot. h Effects of p53 expression on bax promotor activity in FLS as measured by luciferase assay. non, blank control. mock, mock transfection. sc, scrambled RNA control

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