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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Granulocyte macrophage colony-stimulating factor receptor α expression and its targeting in antigen-induced arthritis and inflammation

Fig. 2

Effect of granulocyte macrophage colony-stimulating factor receptor α (GM-CSFRα), TNF and IL-6 blockade on myeloid cell populations in antigen-induced arthritis (AIA). a Representative FACS plots showing the gating strategy used to identify CD45+ myeloid populations in the AIA knee joint. F4/80intSSchi eosinophils (Eos), F4/80+CD11c+MHCII+ monocyte-derived dendritic cells (Mo-DCs) (R1), F4/80+CD11c-MHCII+ macrophages (Macs) (R2), F4/80+CD11c-MHCII- macrophages (R3), F4/80-CD11c+ MHCII+ conventional dendritic cells (cDCs), F4/80-CD11b+SScint Gr-1+ neutrophils (Neutro) and F4/80-CD11b+SScloGr-1+/- monocytes (Mo). b-d AIA-primed mice were treated with PBS, CAT-004 isotype monoclonal antibody (mAb) or CAM-3003 mAb on day -1 and day 2. Cells were harvested at day 5 post AIA induction and synovial populations analysed. b GM-CSFRα expression on myeloid cell populations. c Total CD45+ cells and myeloid cell populations. d MHCII- macrophages, MHCII+ macrophages and Mo-DCs as a percentage of F4/80+ cells. e-f AIA-primed mice were treated with anti-TNF mAb, anti-IL-6 mAb or IgG1 isotype mAb on day -1 and day 2. Cells were harvested at day 5 post AIA induction and synovial populations analysed. e Total cells and myeloid cell populations. f Number of Gr-1+ and Gr-1- cells in the different F4/80+ mononuclear phagocyte system (MPS) cell populations. Data are expressed as mean ± SEM; n = 10–16 mice/group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, CAT-004 vs. CAM-3003, or anti-TNF or anti-IL-6 vs. IgG1

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