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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Granulocyte macrophage colony-stimulating factor receptor α expression and its targeting in antigen-induced arthritis and inflammation

Fig. 5

Granulocyte macrophage colony-stimulating factor receptor α (GM-CSFRα) blockade suppresses generation of monocyte-derived dendritic cells (Mo-DCs) from donor monocytes in the antigen-induced peritonitis (AIP) cavity. CD115+ MacGreen bone marrow cells were adoptively transferred intraperitoneally (i.p.) to C57BL/6 wild-type (WT) mice on day 2 following AIP induction. C57BL/6 mice were treated i.p. with CAT-004 or CAM-3003 at the same time as the CD115+ cell transfer. Peritoneal exudate cells (PECs) were harvested on day 3 and day 4 post AIP induction, i.e., one or two days, respectively, following CD115+ cell transfer and monoclonal antibody (mAb) treatment. a Total cells, monocytes/macrophages (CD11c-MHCII+/-), Mo-DCs and conventional dendritic cells (cDCs) (host and donor cells). b Percentage of CD115+CD11b+ cells that are Ly6C+ vs. Ly6C- (host and donor cells). c GM-CSFRα expression (mean fluorescence intensity (MFI)) on CD115+CD11b+ cells (host and donor cells). d Total numbers of donor cells recovered. e Numbers of donor monocytes/macrophages (CD11c-MHCII+/-) and Mo-DCs recovered. f Percentage of recovered donor cells that are monocytes/macrophages (CD11c-MHCII+/-) and Mo-DCs. g Percentage of recovered donor cells that are Ly6C+ vs. Ly6C-. h GM-CSFRα expression (MFI) on recovered donor cells. Data are expressed as mean ± SEM; n = 8 mice/group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, CAM-3003 vs. CAT-004; ## p < 0.01, CAM-3003 vs. CAT-004

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