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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: CD14brightCD16+ intermediate monocytes are induced by interleukin-10 and positively correlate with disease activity in rheumatoid arthritis

Fig. 5

Inhibition of CD16 expression on monocytes by blockade of IL-10 receptors. a A representative of mean fluorescence intensity shows surface expression of IL-10 receptors using allophycocyanin (APC)-conjugated anti-IL-10 receptor and rat IgG2aκ antibodies on CD14brightCD16- monocytes. b CD14brightCD16- monocytes from healthy volunteers (n = 4), stimulated with various concentrations of IL-10 (0, 1, 10, 25 ng/mL) for 14 hours. c CD14brightCD16- monocytes from healthy volunteers stimulated with IL-10 (25 ng/mL), anti-IL-10 receptor antibody (5 μg/mL), and/or rat IgG2aκ (5 μg/mL). Effect of IL-10 and anti-IL-10 receptor antibody (5 μg/mL) on CD16 expression of monocytes incubated for 14 hours is shown. The median of CD16 expression in monocytes was 0.06% and 0.13% when incubated with nothing or IL-10 + anti-IL-10 receptor, respectively. Bars (b, c) show the median ± SD. Statistical analysis, Dunn’s test

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