Fig. 6

Blocking of semaphorin 7A (Sema7A) attenuates severity of collagen-induced arthritis (CIA) in mice. a Histological examination of a mouse ankle joint on day 50 after treatment with anti-Sema7A antibody. Sections were stained with hematoxylin and eosin. Original magnification × 400. b and c Average arthritis scores and paw swelling of mice with CIA. Mice (n = 8 per group) were immunized with collagen type II, randomized, and, at onset of disease (clinical score of 4), anti-Sema7A or control antibody (Ab) (12 μg/mouse) was administered intraperitoneally on days 1, 10, 20, 30, and 40 (n = 8 mice per group). Data are representative of three independent experiments. d Average histologic scores of paw sections on day 50 (n = 8 mice per group). e Serum samples were collected for expression of tumor necrosis factor-α (TNF-α), interleukin-17 (IL-17), and IL-6 by enzyme-linked immunosorbent assay on day 50 (n = 8 mice per group). Data were compiled from three independent experiments. Values are mean ± SEM. **P < 0.01