Fig. 2
From: Persistent stromal fibroblast activation is present in chronic tendinopathy
Expression of podoplanin (PDPN) mRNA and protein in healthy and diseased tendon cells after IL-1β treatment. Tendon cells were derived from healthy hamstring (n = 5 donors) and diseased supraspinatus tendons (n = 5 donors). Tendon cells were treated with medium containing vehicle only or IL-1β (10 ng/ml-1) for 24 hours. PDPN mRNA expression (a) and PDPN protein (b) are shown in healthy and diseased tendon cells after vehicle and IL-1β treatments. c Representative fluorescence-activated cell sorting (FACS) contour plots for PDPN from healthy and diseased tendon cells after vehicle and IL-1β treatments gated on CD45-CD34- cells. Bar shows median values. Statistically significant differences were calculated using the Kruskal-Wallis test with the pairwise post hoc Mann-Whitney U test; *p < 0.05, **p < 0.01, ***p < 0.001