Fig. 5
From: Persistent stromal fibroblast activation is present in chronic tendinopathy
Effects of IL-1β on podoplanin (PDPN), CD106 and NF-κB genes in healthy and diseased tendon cells over time. Tendon cells were derived from healthy hamstring (n = 4 donors) and diseased supraspinatus tendons (n = 4 donors). Cell lysates were sequentially harvested at day 0 (pre-stimulation), day 1 (24 hours after IL-1β treatment, 10 ng/mL-1) and days 2, 3, 4 and 5 when cells were incubated in cytokine-free medium containing vehicle only. mRNA expression is shown for PDPN (a), CD106 (b), IL6 (c), IL8 (d), STAT-1 (e) and IFIT1 (f). Data are mean ± SEM. Statistically significant differences were calculated using the pairwise Mann-Whitney U test for healthy and diseased cells at each time point; *p < 0.05