Fig. 3

BCP crystal-induced IL-1 production occurs via lipid raft formation in primary macrophages. LPS-primed human macrophages were treated with a–c an Fc receptor blocking antibody (5 μg/ml) or d–f M-βCD (10 mM) for 1 h prior to BCP crystal stimulation for 6 h. Cell supernatants were assessed for IL-1β, IL-1α, and TNF-α by ELISA. Results indicate mean (± SEM) of three independent experiments. ***P < 0.001