Fig. 5
BCP crystals induce expression of damage-associated molecules and MMPs in primary human macrophages in a Syk- and PI3K-dependent manner. Human macrophages (0.5 × 106 cells/well) were stimulated with BCP crystals (50 μg/ml) for 3, 6, and 24 h, and mRNA levels of a S100A8, b S100A12, c MMP1, d MMP2, e MMP9, and f TIMP1 were analysed by real-time PCR. Alternatively, supernatants were harvested; whole cell lysates (WCL) were prepared and both were analysed for the presence of g S100A8, h S100A12, and i MMP1 protein by immunoblotting. Representative blots of three independent experiments are shown. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001