Fig. 6

BCP crystals induce expression of S100A8, S100A12, and MMP1 in primary macrophages in a Syk-, PI3K-, and ERK-dependent manner. Human macrophages were pre-treated with a piceatannol (50 μM, 100 μM), b LY294002 (25 μM, 50 μM), or c PD98059 (5 μM, 10 μM) for 30 mins prior to stimulation with BCP crystals for 24 h. mRNA levels of S100A8, S100A12, MMP1, and TIMP1 were analysed by real-time PCR. Results indicate mean (± SEM) of three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001