Identification of potential saliva and tear biomarkers in primary Sjögren’s syndrome, utilising the extraction of extracellular vesicles and proteomics analysis

Table 4 Highly upregulated proteins in EVs isolated from stimulated whole saliva from patients with pSS

Number	Gene	Related protein^a	Replicates (pSS : C)	Spectral counts (pSS : C)	Classification and function^b
1	APMAP	Adipocyte plasma membrane-associated protein	7 : 0	26 : 0	Enzyme; adipocyte differentiation
2	GNA13	Guanine nucleotide-binding protein subunit alpha-13	6 : 0	11 : 0	G-protein; transmembrane signalling
3	WDR1	WD repeat-containing protein 1	6 : 0	11 : 0	Regulatory protein; disassembly of actin filaments
4	SIRPA	Tyrosine-protein phosphatase non-receptor type substrate 1 (PTPN1)	6 : 0	9 : 0	Glycoprotein; innate immunity (NK cells), dendritic cell inhibition
5	LSP1	Lymphocyte-specific protein 1	5 : 0	9 : 0	Actin-binding protein; neutrophil activation, chemotaxis

^aThe five most upregulated proteins in extracellular vesicles isolated from whole saliva from patients with primary Sjögren’s syndrome (pSS) deviating in replicates, i.e. number of individuals (frequency), and spectral counts, as identified by proteomics analysis and Scaffold (v 4.4.6, http://www.proteomesoftware.com/products/scaffold/). ^bClassification and functions of the proteins presented were identified using publicly available databases, such as UniProt (http://www.uniprot.org). C controls, NK natural killer

ISSN: 1478-6362