Fig. 4
From: Lapachol, a compound targeting pyrimidine metabolism, ameliorates experimental autoimmune arthritis
Immunomodulatory effects of LAP on antigen-induced arthritis (AIA). Methylated bovine serum albumin (mBSA)-immunized C57BL/6 mice were treated orally with lapachol (LAP) (10 mg/kg) once a day over 9 days, beginning 12 days after the first immunization. On day 21 after the first immunization, mice were challenged with an intra-articular injection of 30 μg mBSA. a Leucocyte infiltration into the knee joint analyzed 6 h after mBSA challenge. b, c A pool of cell suspension of draining lymph nodes (inguinal) and spleen from naive or mBSA-immunized mice treated or not with LAP stimulated with mBSA (100 μg/ml) for 96 h. Production of interleuking-17A (IL-17A) (b) and interferon gamma (IFN-γ) (c) by splenic and draining lymph node cells in response to mBSA stimulation measured by ELISA in the culture supernatant. Data represent mean ± SEM, naive (n = 8), vehicle (n = 12), and LAP (n = 14). ***P < 0.001. N.D. not determined