Fig. 2

EBV lytic genes modulate the expression of innate immune mediators, IFN-inducible genes, and proinflammatory cytokines in EBV-p2089-infected monocytes. a,c mRNA expression of indicated innate immune mediators and cytokines was evaluated by quantitative PCR in negative selected CD14/CD16 monocytes infected with EBV-p2089, 5 days PI. Results are expressed as the fold-change induction normalized by one of the mock infected healthy controls. 18S ribosomal RNA levels used as internal control. Bars represent the mean ± SEM. b Western blot analysis was performed to determine IRF7 protein (phosphorylated and total) levels in cell lysates of mock- and EBV-infected monocytes from one representative dcSSc patient and HD, 5 days PI. β-actin was used as loading control. Fold-changes shown on the graph are normalized to mRNA expression by one of the mock infected healthy controls. Bars represent mean ± S.E.M. The two-tailed t test was used for statistical analysis. dcSSc diffuse cutaneous systemic sclerosis, EBV Epstein-Barr virus, HD healthy donor, IL interleukin, Siglec1 sialic acid-binding Ig-like lectin 1 Siglec1/CD169, TNF tumor necrosis factor