Fig. 3

EBV lytic genes are expressed in THP-1 cells. THP-1 cells were infected with EBV. Total RNA and proteins were harvested at indicated time points, processed, and analyzed by quantitative PCR and immunofluorescence. a Detection of EBV-p2089-GFP in THP-1 after 1 h PI (left: phase-contrast light microcopy, scale bar = 0.1 mm). b Double-indirect immunofluorescence staining of THP-1 cells co-stained with anti-EBV/gp-350 antibodies. Diaminidino-2-phenylindole (DAPI) was used as counterstaining for the nuclei. Insert: Higher magnification view for detail (arrow) Original magnification 10×; scale bar = 10 μm. c mRNA expression of indicated genes and proteins in mock-infected and EBV-infected THP-1 cells at indicated time points. Data are expressed as the fold-change normalized to mRNA expression in a mock-infected sample for each time point. Bars represent mean ± SEM from three separate experiments. P values calculated using two-tailed t test; *P < 0.05, **P < 0.01. EBV Epstein-Barr virus, PI post-infection