Fig. 4

TLR8 modulation during EBV infection in THP-1 cells at different time points. THP-1 cells were infected with EBV. Total RNA and proteins were harvested at indicated time points, processed, and analyzed by quantitative PCR and Western blot. a TLR8 mRNA expression in mock-infected and EBV-infected THP-1 cells at indicated time points. Data are expressed as the fold-change normalized to mRNA expression in a mock-infected sample for each time point. Bars represent mean ± SEM from three separate experiments. P values calculated using two-tailed t test; *P < 0.05. b Western blot analysis was performed to determine TLR8 and IRF7 protein (phosphorylated and total) levels in cell lysates at indicated time points post-infection (PI). β-actin was used as loading control. EBV Epstein-Barr virus, TLR Toll-like receptor