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Fig. 9 | Arthritis Research & Therapy

Fig. 9

From: Epstein-Barr virus lytic infection promotes activation of Toll-like receptor 8 innate immune response in systemic sclerosis monocytes

Fig. 9

EBV-lytic proteins are expressed in dcSSc circulating monocytes and dcSSc skin macrophages. Immunofluorescence and Western blot analysis of CD14/CD16 monocytes freshly purified from PBMCs by negative selection, and immunohistochemistry of skin sections. a, c Double immunofluorescence shows the presence of EBV-BFRF1 antigen (green) in CD14+ (red), and EBV-gp-350-220 (red) in CD16+ (green) from two representative diffuse cutaneous systemic sclerosis (dcSSc) patient and two healthy donors (HD). Green arrows indicate monocytes expressing EBV/lytic proteins. Original magnification 60×. Scale bar = 10 μm. b Western blot analysis of EBV-BFRF1 and EBV-BFLF2 lytic proteins in cell lysates of dcSSc and HD monocytes; B95-8/EBV-expressing cells and 293 were used as positive and negative control, respectively. β-actin used as loading control. d Representative immunohistologic images of CD163+ macrophages (brown) and EBV/lytic protein Zebra + (blue) cells in skin sections from dcSSc and HD. Original magnification 2× (upper panels) and 10× (inserts and lower panels). The arrows indicate monocytes represented in the high magnification inserts. Scale bars = 5 μm

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