Fig. 1

Analysis of lymphocyte subpopulations by flow cytometry: gating strategy. A Gating strategy for B lymphocytes; a expressed as a single parameter of CD19 and scatter; b T2/Breg (CD24^hiCD38^hi); c CD24^hiCD27⁺ Breg. B Gating strategy for T lymphocytes; a CD3⁺CD4⁺; b Treg (CD25^hiCD127^low); c CD4⁺45RA⁻; d Th17 (CD45RA⁻CD161⁺CCR6⁺). Breg B regulatory cells, Treg T regulatory cells, Th17 T helper 17