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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Structural cartilage damage attracts circulating rheumatoid arthritis synovial fibroblasts into affected joints

Fig. 2

Tissue extravasation of intravenously injected 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide–labeled human tumor necrosis factor–transgenic (hTNFtg) synovial fibroblasts (SFs). a In vivo coregistration of X-rays and fluorescent signals (cyanine 7 [Cy.7] spectrum) showed an accumulation of hTNFtg SFs in the articular and periarticular zones of the hind legs and liver over time. b Quantification of fluorescent signals revealed an increase in intensity up to 24 h after intravenous (IV) injection of hTNFtg SFs in the regions of interest, such as the juxta-articular areas of femora and tibiae. ce In vivo and ex vivo fusion images of the skeleton and internal organs with quantification of the fluorescent signals. At the time of maximal hTNFtg SF tissue extravasation 24 h after IV injection, detailed preparation and imaging of the different tissues revealed cells additionally in the lungs, liver, spleen, and the lower part of the spinal column and forelegs. The values represent the mean ± SEM for three animals. l/s lumbosacral

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