Fig. 6

Re-stimulation of fibroblast-like synoviocytes (FLS) with TNFα induces prolonged nuclear localization of RelA. FLS were seeded onto chamber slides and stimulated (stim) with 10 ng/mL TNFα for the times indicated (upper images), or stimulated with TNFα for 24 h, washed and rested for 24 h, then re-stimulated for the times indicated (lower images). RelA was detected by immunfluorescence microscopy. The far right images in each row indicate isotype controls for the primary antibody, with 4′,6-diamidino-2-phenylindole (DAPI) staining of nuclei shown in red. Combined RelA and DAPI images are shown in Additional file 1: Figure S1. Images are representative of four experiments using different FLS lines from rheumatoid arthritis