Fig. 4

Adoptive transfer in CIA mice. Passive CIA was induced by intravenous transfer of sorted CD4⁺CD28⁻OX40⁻ (a, n = 4), CD4⁺CD28⁺OX40⁻ (b, n = 4), CD4⁺CD28⁻OX40⁺ (c, n = 5), or CD4⁺CD28⁺OX40⁺ (d, n = 4) T-cell subsets. CIA mice that received transfers with CD4⁺CD28⁻OX40⁻ and CD4⁺CD28⁻OX40⁺ T cells had aggravated arthritis development compared with control CIA mice (n = 4). CD4⁺CD28⁺OX40⁻ and CD4⁺CD28⁺OX40⁺ T cells did not significantly affect arthritis development compared with control CIA mice. The arthritis scores of different groups of recipient mice are shown as the mean ± SD. e H&E histological stains (×200 original magnification) and micro-computed tomographic analysis of representative ankle sections from the experiments shown in a, b, c, and d. CIA Collagen-induced arthritis, H&E Hematoxylin and eosin