Fig. 3
Detection of anti-pp65 and anti-nuclear reactivity from pp65386-403, pp65422-439, streptavidin-complement C3 (SA-C3d) or PBS-immunized serum. The IgG against pp65386-439 and HEK293T extract from pp65386-403 (n = 9), pp65422-439 (n = 9), SA-C3d (n = 5) or PBS (n = 5)-immunized serum at 0, 4, 8 and 14 weeks post immunization were performed at 1:250 dilution. a ELISA for anti-pp65386-403 and anti-pp65422-439 reactivity against pp65386-439 peptide. b ELISA for anti-HEK293T reactivity against total HEK293T lysate. c HEp-2 substrate slides were used for detection of anti-nuclear antibodies. Serum 8 weeks post immunization was 100× diluted for anti-nuclear antibodies (ANA) stain. Nuclear patterns of speckle (b1, b11), nuclear rim (b2, b12), chromatin/nucleosome (b3, b13), centriole (b4, b14), mitotic spindle type I (MSA-I) (b5, b15), MSA-II (b6, b16), nuclear dot (b7, b17) and nuclear envelope (b8, b18) were revealed in serum from pp65422-439-immunized mice. Cytoplasmic response (b9, b19) was detected in serum from pp65386-403-immunized and pp65422-439-immunized mice. Nuclear reactivity was not found in PBS, SA-C3d or pp65386-403-immunized mice (b10, b20). White arrowheads indicate the patterns of nuclear or cytoplasmic response. Data are presented as the mean ± SEM of three independent experiments. w weeks post immunization, O.D. optical density