Fig. 4
Detection of serum anti-double-stranded DNA (anti-dsDNA) antibody and immunoglobulin deposition in glomeruli from immunized mice. a ELISA for anti-dsDNA activity with serum at 1:250 dilution. b Isotyping of anti-dsDNA antibody for pp65422-439 immunized mice (n = 9) at 8 weeks post immunization. Serum was used at 1:250 dilution. dsDNA(+) seropositive for dsDNA, dsDNA(-) seronegative for dsDNA. c Representatives of C. luciliae stain by serum from pp65422-439 (c1), pp65386-403 (c2), streptavidin-complement 3d (SA-C3d) (c3) or PBS (c4) immunized animals at 8 weeks post immunization at 1:80 dilution. White arrowheads indicate dsDNA-positive stains. d Kidney sections from pp65422-439- immunized mice were stained with fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG (d1), IgM (d2) and C3 (d3). Immunoglobulin deposition was not observed in glomeruli from SA-C3d-immunized (d4) or PBS-immunized mice. e Kidney sections from pp65422-439-immunized mice were stained with FITC-conjugated anti-mouse IgG1 (e1), IgG2a (e2) IgG2b (e3) and IgG3 (e4). Kidneys were collected at 22 weeks of mice age. Data are presented as the mean ± SEM of three independent experiments. w weeks post immunization, O.D. optical density