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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: B cell epitope of human cytomegalovirus phosphoprotein 65 (HCMV pp65) induced anti-dsDNA antibody in BALB/c mice

Fig. 5

Antibody activity against pp65422-439-derived decapeptides and double-stranded DNA (dsDNA) from anti-pp65422-439 antibodies in human systemic lupus erythematosus (SLE), immunized mice serum and monoclonal antibodies from pp65422-439-immunized mice. a Representatives of nine overlapping pp65422-439-derived decapeptides. Each peptide is shifted by one amino acid. ELISA for anti-decapeptide reactivity with four monoclonal antibodies (b) and anti-pp65422-439 antibody (c) from SLE-dsDNA(+) serum: 100× diluted normal serum and flow-through were used as negative controls. d ELISA for IgG against pp65422-439 and dsDNA with monoclonal antibodies and 100× diluted normal serum. e Representatives of C. luciliae stain by P1 (e1), P2 (e2), P3 (e3) or P4 (e4) monoclonal antibodies, respectively. White arrowheads indicate the positive stains. Data are presented as the mean ± SEM of three independent experiments. O.D. optical density

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