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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Glutaminase 1 plays a key role in the cell growth of fibroblast-like synoviocytes in rheumatoid arthritis

Fig. 3

Glutamine is required for the proliferation of RA-FLS. a RA-FLS proliferation was determined using the BrdU assay 96 h after transfection with HK2, MCT4, PDK1, GLS1, or SC siRNA (n = 5). Each experiment was performed in quintuplicate. Bars indicate the mean ± SEM. *P < 0.05, **P < 0.01 versus SC by Student’s t test. b RA-FLS proliferation was determined using the BrdU assay 96 h after culturing in medium with both Glc and Gln, or in medium without Glc or Gln (n = 7). Each experiment was performed in quintuplicate. Bars indicate mean ± SEM. *P < 0.05, **P < 0.01. Glc glucose, Gln glutamine, GLS glutaminase, HK hexokinase, MCT monocarboxylate transporter, PDK pyruvate dehydrogenase kinase, RA-FLS fibroblast-like synoviocytes from rheumatoid arthritis patients, SC control scrambled, siRNA small interfering RNA

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