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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis

Fig. 1

Human CD4+CD25LAG3+ T cells suppressed antibody production. a Gating strategy of fluorescence-activated cell sorting analysis for CD4+CD25LAG3+CD45RA T cells (LAG3+ Tregs) and CD4+CD25+CD127dimCD45RA T cells (CD25+ Tregs). b IL-10, IFN-γ, and IL-17A protein levels in the culture supernatants after 3 days of incubation of the indicated T-cell subsets was determined by ELISA (n = 6). c B cells and TFH cells were cocultured with the indicated subsets for 12 days in the presence of SEB stimulation, and total IgG, IgM, and IgA production was determined by ELISA (n = 6). d B cells and TFH cells were cocultured with CD4+CD25LAG3+CD49b T cells for 12 days in the presence of SEB stimulation, and total IgG was determined by ELISA (n = 3). All error bars represent SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. All P values were calculated by one-way analysis of variance and Tukey’s multiple-comparisons test. CD Cluster of differentiation, ELISA Enzyme-linked immunosorbent assay, IFN-γ Interferon-γ, Ig Immunoglobulin, IL Interleukin, LAG3 Lymphocyte activation gene 3, RA Rheumatoid arthritis, SEB Staphylococcal enterotoxin B, T FH Follicular helper T cell, Treg Regulatory T cell

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