Fig. 4

Prophylactic URB597 inhibits demyelination of joint nerves 14 days after MIA injection. Representative electron micrographs of axons found in saphenous nerves taken at day 14 from animals with a (a) saline-injected knee, (b) MIA-injected joint treated with vehicle (days 0–3) and (c) MIA-injected joint treated with URB597 (0.3 mg/kg, days 0–3). Myelin thickness is noticeably less in OA joint nerves and this demyelination is prevented by prophylactic URB597 treatment. d G-ratio calculations showing that MIA causes saphenous axonal demyelination which is prevented by URB597 treatment. Scale bar = 6 μm. *P < 0.05 one-way ANOVA with Tukey’s post-hoc test; n = 5–7. Data presented as mean ± SEM. MIA monoiodoacetate