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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Mir-155 is overexpressed in systemic sclerosis fibroblasts and is required for NLRP3 inflammasome-mediated collagen synthesis during fibrosis

Fig. 4

miR-155 regulates fibrosis via interleukin-1 (IL-1) and IL-1 induces miR-155. a miR-155KO fibroblasts were transduced with the miR-155 expression vector or the control (Ctl) vector. Some of the dishes received 10 μM bleomycin (Bleo) at 0 h and some of the cells also received 50 ng/ml IL-1 receptor antagonist (IL-1RA) at 0 and 24 h. Media was recovered after 48 h and hydroxyproline was measured (n = 9 replicates). b Dose-dependent induction of miR-155 in fibroblasts (n = 7 replicates). c Graphical representation of transforming growth factor beta (TGF-β) levels in miR-155KO and B6 cells ± bleo. d Representative Western blot (one of three samples independently tested). Data are presented as averages + SEM using the Mann-Whitney t test. ns Not significant

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