Fig. 4

Histological analysis of arthritic lesions in Cxcl10 ^–/– and Cxcr3 ^–/– mice with CAIA. Tissue sections were obtained from paw joints of the same mice as in Fig. 3 on day 12 after arthritic induction. Representative images of Safranin O, H&E, and TRAP staining (a, c) and quantification of histological analysis (b, d) of joint tissue sections from WT and Cxcl10 ^–/– mice (a, b) and WT and Cxcr3 ^–/– mice (c, d) with CAIA. Fourth panel (a, c) is a higher magnification image of the boxed area in the third panel. Scale bar, 400 μm (first–third panels) and 100 μm (fourth panel). Data represent mean ± SEM of results in five samples of staining (n = 5). *P < 0.001 vs WT mice by unpaired Student’s t test. WT wild-type, CXCL10 C-X-C motif chemokine 10, Cxcr3 CXC chemokine receptor 3, H&E hematoxylin and eosin, TRAP tartrate-resistant acid phosphatase (Color figure online)