Fig. 6

Reduction of osteoclastogenic cytokine levels in serum and spleen of Cxcl10 ^–/– and Cxcr3 ^–/– mice with CAIA. a, b Serum was collected from the same WT, Cxcl10 ^–/– (a), and Cxcr3 ^–/– (b) mice as in Fig. 3 on day 12. Serum levels of CXCL10, RANKL, TNFα, IL-6, and CTX were measured by ELISA. Data represent mean ± SEM. *P < 0.001 by one-way ANOVA followed by Dunnett’s test. c, d Spleens were collected from the same WT, Cxcl10 ^–/– (c), and Cxcr3 ^–/– (d) mice as in Fig. 3 on day 12. RANKL, TNFα, IL-6, and CXCL10 mRNA levels were analyzed by real-time PCR. Data represent mean ± SEM of results in five samples (n = 5). *P < 0.001 vs WT mice by unpaired Student’s t test. WT wild-type, CXCL10 C-X-C motif chemokine 10, Cxcr3 CXC chemokine receptor 3, CAIA collagen antibody-induced arthritis, RANKL receptor activator of nuclear factor kappa-B ligand, TNFα tumor necrosis factor α, IL-6 interleukin 6, CTX C-terminal telopeptide, ND not detected