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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Therapeutic effect of a novel histone deacetylase 6 inhibitor, CKD-L, on collagen-induced arthritis in vivo and regulatory T cells in rheumatoid arthritis in vitro

Fig. 3

Increased cytotoxic T-lymphocyte antigen-4 (CTLA-4) expression by CKD-L in Treg cells from C57BL/6 mice (n = 3). CD4+CD25 T cells were isolated from splenocytes of C57BL/6 mice. CD4+CD25 T cells were incubated with vehicle or HDAC6 inhibitors (1 to 10 μM) in the presence of antiCD3/CD28 beads and recombinant TGF-β2 in a 48-well plate for 6 days. CTLA-4 expression in CD4+CD25+Foxp3+ T cells was analyzed by mean fluorescence intensity (MFI; mean ± SEM) using flow cytometry. *p < 0.05, **p < 0.001, vs vehicle

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