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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Therapeutic effect of a novel histone deacetylase 6 inhibitor, CKD-L, on collagen-induced arthritis in vivo and regulatory T cells in rheumatoid arthritis in vitro

Fig. 4

Effect of CKD-L in the suppression assay using regulatory T (Treg) cells and effector T (Teff) cells from C57BL/6 mice (n = 3). Treg cells were mixed with eFluor®670-labeled Teff cells at ratios of 4:1 and 0:1. The mixed cells were incubated with vehicle or HDAC6 inhibitor (1 to10 μM) in the presence of antiCD3/CD28 beads for 4 days. a The proliferation of Teff cells was assessed by eFluor®670 dilution using flow cytometry (representative data). b Suppression ratio (fold inhibition of cell proliferation by HDACi vs. vehicle) at a ratio of Treg:Teff = 1:4. *p < 0.01, **p < 0.001, vs vehicle

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