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Fig. 7 | Arthritis Research & Therapy

Fig. 7

From: Therapeutic effect of a novel histone deacetylase 6 inhibitor, CKD-L, on collagen-induced arthritis in vivo and regulatory T cells in rheumatoid arthritis in vitro

Fig. 7

Effects of CKD-L on cytokine mRNA expression in PBMC from RA patients (n = 3). PBMC from RA patients were cultured in a 48-well plate for 24 h in the presence of lipopolysaccharide (LPS; 100 ng/ml) and HDAC inhibitors at different concentrations (0.01 to 5 μM). Total RNA was extracted from harvested cells and cDNA was synthesized by RT-PCR. Tumor necrosis factor (TNF) (a) and interleukin-10 (IL-10) (b) mRNA expression levels were analyzed by real-time PCR. *p < 0.001, **p < 0.01, vs LPS

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